Biophysical backgrounds of the FIRe technique are described in peer-reviewed literature (Gorbunov and Falkowski 2021; 2022).
Fluorescent measurements of absolute photosynthetic rates (i.e., photosynthetic electron transport rates, ETR, and primary production rates) are based on the analysis of variable fluorescence signals as a function of irradiance (PAR) (Gorbunov and Falkowski 2022). The classic approach, called the amplitude-based method, relies on recording changes in fluorescence amplitudes and is subject to many errors and uncertainties.
In contrast, the new kinetic approach relies on instantaneous measurements of fluorescence relaxation kinetics, which reflects the absolute rates at which the absorbed light energy is used for carbon fixation (Gorbunov and Falkowski, 2021).
Fluorescent measurements of absolute photosynthetic rates (i.e., photosynthetic electron transport rates, ETR, and primary production rates) are based on the analysis of variable fluorescence signals as a function of irradiance (PAR) (Gorbunov and Falkowski 2022). The classic approach, called the amplitude-based method, relies on recording changes in fluorescence amplitudes and is subject to many errors and uncertainties.
In contrast, the new kinetic approach relies on instantaneous measurements of fluorescence relaxation kinetics, which reflects the absolute rates at which the absorbed light energy is used for carbon fixation (Gorbunov and Falkowski, 2021).
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Comparison of classic amplitude-based (B) and kinetic (C) measurements of photosynthetic rates, in relation to growth rates.
The use of kinetic analysis dramatically improves the accuracy of fluorescence-based measurements of photosynthetic rates , as evident from vastly superior correlation with growth rates. Data from (Gorbunov and Falkowski, 2021).
The kinetic analysis capability is unique for mini-FIRe technology and is not available in other instruments, such as FRR fluorometers.
The use of kinetic analysis dramatically improves the accuracy of fluorescence-based measurements of photosynthetic rates , as evident from vastly superior correlation with growth rates. Data from (Gorbunov and Falkowski, 2021).
The kinetic analysis capability is unique for mini-FIRe technology and is not available in other instruments, such as FRR fluorometers.